PrimePCR™ SYBR® Green Assay: APOB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030812

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: APOB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: APOB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016796
Assay Design:   Intron-spanning
Chromosome Location:   2:21251341-21252587question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000233242 ENST00000399256 ENST00000535079

Gene Information

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA) resulting in the creation of a stop codon and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia normotriglyceridemic hypobetalipoproteinemia and hypercholesterolemia due to ligand-defective apoB diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq Jul 2008]

Gene Symbol:   APOB
Gene Name:   apolipoprotein B (including Ag(x) antigen)
Aliases:   FLDB, LDLCQ4
RefSeq:   NC_000002.11 NG_011793.1 NT_022184.15
Ensembl:   ENSG00000084674
Entrez:   338
Chromosome Mapping:   2p24-p23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.760000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download