PrimePCR™ SYBR® Green Assay: MED16, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030858

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MED16, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MED16, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016866
Assay Design:   Intron-spanning
Chromosome Location:   19:891112-893209question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000325464 ENST00000312090 ENST00000395808 ENST00000269814 ENST00000424039 ENST00000534906 ENST00000537596 ENST00000540679 ENST00000538572 ENST00000541440

Gene Information

Description Not Available

Gene Symbol:   MED16
Gene Name:   mediator complex subunit 16
Aliases:   DRIP92, THRAP5, TRAP95
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000175221
Entrez:   10025
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.400000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download