PrimePCR™ SYBR® Green Assay: SLC19A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0031109


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PrimePCR™ PreAmp for SYBR® Green Assay: SLC19A2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SLC19A2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0017235
Assay Design:		Intron-spanning
Chromosome Location:		1:169437928-169439228question
Amplicon Length:		144
Splice Variants Targeted:		ENST00000236137 ENST00000367804 ENST00000367802

Gene Information

This gene encodes the thiamin transporter protein. Mutations in this gene cause thiamin-responsive megaloblastic anemia syndrome (TRMA) which is an autosomal recessive disorder characterized by diabetes mellitus megaloblastic anemia and sensorineural deafness. [provided by RefSeq Jul 2008]

Gene Symbol:		SLC19A2
Gene Name:		solute carrier family 19 (thiamine transporter), member 2
Aliases:		TC1, THT1, THTR1, TRMA
RefSeq:		NC_000001.10 NG_008255.1 NT_004487.19
Ensembl:		ENSG00000117479
Entrez:		10560
Chromosome Mapping:		1q23.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999000
y-intercept	34.710000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.