PrimePCR™ SYBR® Green Assay: CES3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031177

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CES3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CES3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017346
Assay Design:   Intron-spanning
Chromosome Location:   16:67005143-67006312question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000303334 ENST00000394037 ENST00000543856

Gene Information

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics such as cocaine and heroin and endogenous substrates with ester thioester or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism and may play a role in the blood-brain barrier system. This gene is expressed in several tissues particularly in colon trachea and in brain and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported but the biological validity and/or full-length nature of some variants have not been determined.[provided by RefSeq Jun 2010]

Gene Symbol:   CES3
Gene Name:   carboxylesterase 3
Aliases:   ES31, FLJ21736
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000172828
Entrez:   23491
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.380000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download