PrimePCR™ SYBR® Green Assay: SNRPB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031206

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SNRPB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SNRPB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017385
Assay Design:   Intron-spanning
Chromosome Location:   20:2444508-2448263question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000381342 ENST00000438552 ENST00000303103 ENST00000339610

Gene Information

The protein encoded by this gene is one of several nuclear proteins that are found in common among U1 U2 U4/U6 and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SNRPB
Gene Name:   small nuclear ribonucleoprotein polypeptides B and B1
Aliases:   COD, SNRPB1, Sm-B/B', SmB/B', SmB/SmB', snRNP-B
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000125835
Entrez:   6628
Chromosome Mapping:   20p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.950000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download