PrimePCR™ SYBR® Green Assay: ZC3H4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031364

List Price:    $188.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZC3H4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZC3H4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017616
Assay Design:   Intron-spanning
Chromosome Location:   19:47593366-47597321question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000253048

Gene Information

This gene encodes a member of a family of CCCH (C-x8-C-x5-C-x3-H type) zinc finger domain-containing proteins. These zinc finger domains which coordinate zinc finger binding and are characterized by three cysteine residues and one histidine residue are nucleic acid-binding. Other family members are known to function in post-transcriptional regulation. [provided by RefSeq Aug 2011]

Gene Symbol:   ZC3H4
Gene Name:   zinc finger CCCH-type containing 4
Aliases:   C19orf7, KIAA1064
RefSeq:   NC_000019.9 NG_027798.1 NT_011109.16
Ensembl:   ENSG00000130749
Entrez:   23211
Chromosome Mapping:   19q13.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.160000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download