PrimePCR™ SYBR® Green Assay: ATP7A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031403

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ATP7A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ATP7A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017681
Assay Design:   Intron-spanning
Chromosome Location:   X:77166294-77227217question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000341514 ENST00000343912 ENST00000343533 ENST00000350425 ENST00000400860

Gene Information

This gene encodes a transmembrane protein that functions in copper transport across membranes. This protein is localized to the trans-Golgi network where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. It relocalizes to the plasma membrane under conditions of elevated extracellular copper and functions in the efflux of copper from cells. Mutations in this gene are associated with Menkes disease X-linked cutis laxa and occipital horn syndrome. [provided by RefSeq Oct 2011]

Gene Symbol:   ATP7A
Gene Name:   ATPase, Cu++ transporting, alpha polypeptide
Aliases:   DSMAX, FLJ17790, MK, MNK, SMAX3
RefSeq:   NG_021360.1 NC_000023.10 NG_013224.1 NT_011651.17
Ensembl:   ENSG00000165240
Entrez:   538
Chromosome Mapping:   Xq21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.220000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download