PrimePCR™ SYBR® Green Assay: ALB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031466

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ALB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ALB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017798
Assay Design:   Intron-spanning
Chromosome Location:   4:74279280-74280821question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000295897 ENST00000415165 ENST00000503124 ENST00000509063 ENST00000401494 ENST00000511370 ENST00000430202

Gene Information

Albumin is a soluble monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids fatty acids and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product proalbumin is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq Jul 2008]

Gene Symbol:   ALB
Gene Name:   albumin
Aliases:   DKFZp779N1935, PRO0883, PRO0903, PRO1341
RefSeq:   NC_000004.11 NG_009291.1 NT_022778.16
Ensembl:   ENSG00000163631
Entrez:   213
Chromosome Mapping:   4q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.260000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download