PrimePCR™ SYBR® Green Assay: CLTC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0031476


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PrimePCR™ PreAmp for SYBR® Green Assay: CLTC, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CLTC, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0017813
Assay Design:		Intron-spanning
Chromosome Location:		17:57697444-57721706question
Amplicon Length:		131
Splice Variants Targeted:		ENST00000269122 ENST00000393043

Gene Information

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq Jul 2008]

Gene Symbol:		CLTC
Gene Name:		clathrin, heavy chain (Hc)
Aliases:		CHC, CHC17, CLH-17, CLTCL2, Hc, KIAA0034
RefSeq:		NC_000017.10 NT_010783.15
Ensembl:		ENSG00000141367
Entrez:		1213
Chromosome Mapping:		17q11-qter

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	34.860000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.