PrimePCR™ SYBR® Green Assay: DNAH5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031524

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DNAH5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DNAH5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017886
Assay Design:   Intron-spanning
Chromosome Location:   5:13769636-13770947question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000265104

Gene Information

This gene encodes a dynein protein which is part of a microtubule-associated motor protein complex consisting of heavy light and intermediate chains. This protein is an axonemal heavy chain dynein. It functions as a force-generating protein with ATPase activity whereby the release of ADP is thought to produce the force-producing power stroke. Mutations in this gene cause primary ciliary dyskinesia type 3 as well as Kartagener syndrome which are both diseases due to ciliary defects. [provided by RefSeq Oct 2009]

Gene Symbol:   DNAH5
Gene Name:   dynein, axonemal, heavy chain 5
Aliases:   CILD3, DNAHC5, FLJ46759, HL1, KIAA1603, KTGNR, PCD
RefSeq:   NC_000005.9 NG_013081.1 NT_006576.16
Ensembl:   ENSG00000039139
Entrez:   1767
Chromosome Mapping:   5p15.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.560000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download