PrimePCR™ SYBR® Green Assay: CCRL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0031634


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PrimePCR™ PreAmp for SYBR® Green Assay: CCRL1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CCRL1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0018078
Assay Design:		Intron-spanning
Chromosome Location:		3:132316081-132319280question
Amplicon Length:		105
Splice Variants Targeted:		ENST00000249887 ENST00000424114

Gene Information

The protein encoded by this gene is a member of the G protein-coupled receptor family and is a receptor for C-C type chemokines. This receptor has been shown to bind dendritic cell- and T cell-activated chemokines including CCL19/ELC CCL21/SLC and CCL25/TECK. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq Jul 2008]

Gene Symbol:		CCRL1
Gene Name:		chemokine (C-C motif) receptor-like 1
Aliases:		CC-CKR-11, CCBP2, CCR-11, CCR10, CCR11, CCX CKR, CCX-CKR, CKR-11, PPR1, VSHK1
RefSeq:		NC_000003.11 NT_005612.16
Ensembl:		ENSG00000129048
Entrez:		51554
Chromosome Mapping:		3q22

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	34.660000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.