PrimePCR™ SYBR® Green Assay: KCNIP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: KCNIP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KCNIP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018126
Assay Design:   Intron-spanning
Chromosome Location:   5:170149775-170159893question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000377360 ENST00000328939 ENST00000520740 ENST00000434108 ENST00000411494 ENST00000390656

Gene Information

This gene encodes a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs) which belong to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents and hence neuronal excitability in response to changes in intracellular calcium. Alternative splicing results in multiple transcript variant encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   KCNIP1
Gene Name:   Kv channel interacting protein 1
Aliases:   KCHIP1, MGC95, VABP
RefSeq:   NG_011452.1 NC_000005.9 NT_023133.13 NG_011538.1
Ensembl:   ENSG00000182132
Entrez:   30820
Chromosome Mapping:   5q35.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.710000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download