PrimePCR™ SYBR® Green Assay: FAM149A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031680

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FAM149A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FAM149A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018144
Assay Design:   Intron-spanning
Chromosome Location:   4:187073090-187074842question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000503432 ENST00000504330 ENST00000510790 ENST00000227065 ENST00000502970 ENST00000514153 ENST00000356371 ENST00000389354

Gene Information

Description Not Available

Gene Symbol:   FAM149A
Gene Name:   family with sequence similarity 149, member A
Aliases:   DKFZp564J102, MSTP119
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000109794
Entrez:   25854
Chromosome Mapping:   4q35.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 34.130000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download