PrimePCR™ SYBR® Green Assay: CAPN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0031689


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PrimePCR™ PreAmp for SYBR® Green Assay: CAPN2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CAPN2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0018157
Assay Design:		Intron-spanning
Chromosome Location:		1:223949889-223954097question
Amplicon Length:		122
Splice Variants Targeted:		ENST00000295006 ENST00000433674 ENST00000366869

Gene Information

The calpains calcium-activated neutral proteases are nonlysosomal intracellular cysteine proteases. The mammalian calpains include ubiquitous stomach-specific and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large catalytic subunits associated with a common small regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:		CAPN2
Gene Name:		calpain 2, (m/II) large subunit
Aliases:		CANP2, CANPL2, CANPml, FLJ39928, mCANP
RefSeq:		NC_000001.10 NT_167186.1
Ensembl:		ENSG00000162909
Entrez:		824
Chromosome Mapping:		1q41-q42

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	35.110000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.