PrimePCR™ SYBR® Green Assay: PFKFB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0031766


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PrimePCR™ PreAmp for SYBR® Green Assay: PFKFB2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PFKFB2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0018283
Assay Design:		Intron-spanning
Chromosome Location:		1:207241559-207242826question
Amplicon Length:		124
Splice Variants Targeted:		ENST00000367080 ENST00000367079 ENST00000545806 ENST00000411990 ENST00000541914

Gene Information

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-26-bisphosphate a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-26-bisphosphate and a fructose-26-biphosphatase activity that catalyzes the degradation of fructose-26-bisphosphate. This protein regulates fructose-26-bisphosphate levels in the heart while a related enzyme encoded by a different gene regulates fructose-26-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		PFKFB2
Gene Name:		6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Aliases:		DKFZp781D2217, MGC138308, MGC138310, PFK-2/FBPase-2
RefSeq:		NC_000001.10 NT_167186.1
Ensembl:		ENSG00000123836
Entrez:		5208
Chromosome Mapping:		1q31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	33.990000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.