PrimePCR™ SYBR® Green Assay: IGF2R, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031773

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IGF2R, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IGF2R, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018295
Assay Design:   Intron-spanning
Chromosome Location:   6:160517596-160523630question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000356956

Gene Information

The protein encoded by this gene is a receptor for both insulin-like growth factor 2 (IGF2) and mannose 6-phosphate (M6P). The IGF2 and M6P binding sites are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes the activation of transforming growth factor beta and the degradation of IGF2. While the mouse Igf2r gene shows exclusive expression from the maternal allele imprinting of the human IGF2R gene appears to be polymorphic with only a minority of individuals showing expression from the maternal allele. [provided by RefSeq Oct 2010]

Gene Symbol:   IGF2R
Gene Name:   insulin-like growth factor 2 receptor
Aliases:   CD222, CIMPR, M6P-R, MPR1, MPRI
RefSeq:   NC_000006.11 NG_011785.1 NT_025741.15
Ensembl:   ENSG00000197081
Entrez:   3482
Chromosome Mapping:   6q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.140000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download