PrimePCR™ SYBR® Green Assay: RAD54B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

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PrimePCR™ PreAmp for SYBR® Green Assay: RAD54B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: RAD54B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018589
Assay Design:   Intron-spanning
Chromosome Location:   8:95470631-95479732question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000336148 ENST00000523839 ENST00000297592 ENST00000546218 ENST00000543299

Gene Information

The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54 both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq Jul 2008]

Gene Symbol:   RAD54B
Gene Name:   RAD54 homolog B (S. cerevisiae)
Aliases:   FSBP, RDH54
RefSeq:   NC_000008.10 NT_008046.16 NG_012878.1
Ensembl:   ENSG00000197275
Entrez:   25788
Chromosome Mapping:   8q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download