PrimePCR™ SYBR® Green Assay: IMPDH2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IMPDH2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IMPDH2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0019696
Assay Design:   Intron-spanning
Chromosome Location:   3:49064409-49065193question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000429182 ENST00000326739 ENST00000442157 ENST00000537036

Gene Information

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms suggesting it may play a role in malignant transformation. [provided by RefSeq Jul 2008]

Gene Symbol:   IMPDH2
Gene Name:   IMP (inosine 5'-monophosphate) dehydrogenase 2
Aliases:   IMPD2, IMPDH-II
RefSeq:   NC_000003.11 NG_012091.1 NG_016282.1 NT_022517.18
Ensembl:   ENSG00000178035
Entrez:   3615
Chromosome Mapping:   3p21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.000000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download