PrimePCR™ SYBR® Green Assay: HYOU1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0032511

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HYOU1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HYOU1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0019708
Assay Design:   Intron-spanning
Chromosome Location:   11:118920499-118921814question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000404233 ENST00000529972 ENST00000525859 ENST00000530473 ENST00000536103 ENST00000544701 ENST00000353883 ENST00000535579 ENST00000543287

Gene Information

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors especially in breast tumors and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein which is only 3 amino acids shorter than the mature protein in the ER is thought to have a housekeeping function in the cytosol. In rat this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. [provided by RefSeq Oct 2008]

Gene Symbol:   HYOU1
Gene Name:   hypoxia up-regulated 1
Aliases:   DKFZp686N08236, FLJ94899, FLJ97572, Grp170, HSP12A, ORP150
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000149428
Entrez:   10525
Chromosome Mapping:   11q23.1-q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.010000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download