PrimePCR™ SYBR® Green Assay: MST1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: MST1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MST1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0019722
Assay Design:   Intron-spanning
Chromosome Location:   3:49721815-49722012question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000448220 ENST00000449682

Gene Information

The protein encoded by this gene contains four kringle domains and a serine protease domain similar to that found in hepatic growth factor. Despite the presence of the serine protease domain the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds. [provided by RefSeq Jan 2010]

Gene Symbol:   MST1
Gene Name:   macrophage stimulating 1 (hepatocyte growth factor-like)
Aliases:   D3F15S2, DNF15S2, HGFL, MSP, NF15S2
RefSeq:   NC_000003.11 NG_011438.1 NG_011603.1 NG_016454.1 NT_022517.18
Ensembl:   ENSG00000173531
Entrez:   4485
Chromosome Mapping:   3p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.950000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download