PrimePCR™ SYBR® Green Assay: PDE4DIP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0032692


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PrimePCR™ PreAmp for SYBR® Green Assay: PDE4DIP, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PDE4DIP, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0020241
Assay Design:		Intron-spanning
Chromosome Location:		1:144912236-144915487question
Amplicon Length:		72
Splice Variants Targeted:		ENST00000313382 ENST00000369354 ENST00000369356 ENST00000530740 ENST00000369359 ENST00000369351 ENST00000369349 ENST00000313431 ENST00000529945 ENST00000479408 ENST00000369353

Gene Information

The protein encoded by this gene serves to anchor phosphodiesterase 4D to the Golgi/centrosome region of the cell. Defects in this gene may be a cause of myeloproliferative disorder (MBD) associated with eosinophilia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2010]

Gene Symbol:		PDE4DIP
Gene Name:		phosphodiesterase 4D interacting protein
Aliases:		CMYA2, DKFZp781J054, MGC75440, MMGL
RefSeq:		NC_000001.10 NT_167185.1 NG_027693.1
Ensembl:		ENSG00000178104
Entrez:		9659
Chromosome Mapping:		1q12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	34.760000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.