PrimePCR™ SYBR® Green Assay: WBP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: WBP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: WBP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0020852
Assay Design:   Intron-spanning
Chromosome Location:   2:74685694-74686833question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000233615 ENST00000393972 ENST00000409737 ENST00000428943

Gene Information

The globular WW domain named for the conserved tryptophan residues in the protein motif present in various structural and regulatory proteins is known to play a role in the mediation of protein-protein interactions. This gene encodes a ligand of the WW domain of the Yes kinase-associated protein. Readthrough transcription of the neighboring upstream gene which encodes INO80 complex subunit B into this gene generates a non-coding transcript. [provided by RefSeq Feb 2011]

Gene Symbol:   WBP1
Gene Name:   WW domain binding protein 1
Aliases:   MGC15305, WBP-1
RefSeq:   NC_000002.11 NG_008922.1 NT_022184.15
Ensembl:   ENSG00000239779
Entrez:   23559
Chromosome Mapping:   2p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994900
y-intercept 37.070000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download