PrimePCR™ SYBR® Green Assay: COX10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: COX10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: COX10, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0020867
Assay Design:   Intron-spanning
Chromosome Location:   17:14095440-14110185question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000261643 ENST00000536205 ENST00000537334

Gene Information

Cytochrome c oxidase (COX) the terminal component of the mitochondrial respiratory chain catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes heme A:farnesyltransferase which is not a structural subunit but required for the expression of functional COX and functions in the maturation of the heme A prosthetic group of COX. This protein is predicted to contain 7-9 transmembrane domains localized in the mitochondrial inner membrane. A gene mutation which results in the substitution of a lysine for an asparagine (N204K) is identified to be responsible for cytochrome c oxidase deficiency. In addition this gene is disrupted in patients with CMT1A (Charcot-Marie-Tooth type 1A) duplication and with HNPP (hereditary neuropathy with liability to pressure palsies) deletion. [provided by RefSeq Jul 2008]

Gene Symbol:   COX10
Gene Name:   COX10 homolog, cytochrome c oxidase assembly protein, heme A: farnesyltransferase (yeast)
Aliases:   Not Available
RefSeq:   NC_000017.10 NG_008034.1 NT_010718.16
Ensembl:   ENSG00000006695
Entrez:   1352
Chromosome Mapping:   17p12

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