This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0032827
PrimePCR™ PreAmp for SYBR® Green Assay: TNFRSF9, Human
PrimePCR™ Template for SYBR® Green Assay: TNFRSF9, Human
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion survival and development of T cells. It can also induce proliferation in peripheral monocytes enhance T cell apoptosis induced by TCR/CD3 triggered activation and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.