PrimePCR™ SYBR® Green Assay: DLD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0032854

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: DLD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DLD, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0020978
Assay Design:   Intron-spanning
Chromosome Location:   7:107546721-107556006question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000205402 ENST00000417551 ENST00000440410 ENST00000437604 ENST00000539590 ENST00000537148

Gene Information

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein also named dihydrolipoamide dehydrogenase is also a component of the pyruvate dehydrogenase complex the alpha-ketoglutarate dehydrogenase complex and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq Jul 2008]

Gene Symbol:   DLD
Gene Name:   dihydrolipoamide dehydrogenase
Aliases:   DLDH, E3, GCSL, LAD, PHE3
RefSeq:   NC_000007.13 NG_008045.1 NT_007933.15
Ensembl:   ENSG00000091140
Entrez:   1738
Chromosome Mapping:   7q31-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.020000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download