PrimePCR™ SYBR® Green Assay: NQO2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0032962


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PrimePCR™ PreAmp for SYBR® Green Assay: NQO2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: NQO2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0021208
Assay Design:		Intron-spanning
Chromosome Location:		6:3006705-3010309question
Amplicon Length:		110
Splice Variants Targeted:		ENST00000426637 ENST00000380472 ENST00000397717 ENST00000338130 ENST00000380441 ENST00000380455 ENST00000380454 ENST00000380430 ENST00000538898

Gene Information

NQO2 (EC 1.10.99.2) is a flavoprotein that catalyzes the 2-electron reduction of various quinones redox dyes and the vitamin K menadione. NQO2 predominantly uses dihydronicotinamide riboside (NRH) as the electron donor (summary by Wu et al. 1997 [PubMed 9367528]).[supplied by OMIM Jul 2010]

Gene Symbol:		NQO2
Gene Name:		NAD(P)H dehydrogenase, quinone 2
Aliases:		DHQV, DIA6, NMOR2, QR2
RefSeq:		NC_000006.11 NT_007592.15
Ensembl:		ENSG00000124588
Entrez:		4835
Chromosome Mapping:		6pter-q12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	35.190000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.