PrimePCR™ SYBR® Green Assay: MTX2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0032971


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PrimePCR™ PreAmp for SYBR® Green Assay: MTX2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MTX2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0021234
Assay Design:		Intron-spanning
Chromosome Location:		2:177194139-177202302question
Amplicon Length:		145
Splice Variants Targeted:		ENST00000249442 ENST00000443241 ENST00000452865 ENST00000392529

Gene Information

The protein encoded by this gene is highly similar to the metaxin 2 protein from mouse which has been shown to interact with the mitochondrial membrane protein metaxin 1. Because of this similarity it is thought that the encoded protein is peripherally associated with the cytosolic face of the outer mitochondrial membrane and that it is involved in the import of proteins into the mitochondrion. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq Jun 2009]

Gene Symbol:		MTX2
Gene Name:		metaxin 2
Aliases:		MGC111067
RefSeq:		NC_000002.11 NT_005403.17
Ensembl:		ENSG00000128654
Entrez:		10651
Chromosome Mapping:		2q31.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	34.870000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.