PrimePCR™ SYBR® Green Assay: CREBBP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033238

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CREBBP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CREBBP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021913
Assay Design:   Intron-spanning
Chromosome Location:   16:3786722-3788608question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000262367 ENST00000543883 ENST00000382070 ENST00000323508

Gene Information

This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB) this gene is now known to play critical roles in embryonic development growth control and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain cysteine-histidine-rich regions and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Feb 2009]

Gene Symbol:   CREBBP
Gene Name:   CREB binding protein
Aliases:   CBP, KAT3A, RSTS
RefSeq:   NC_000016.9 NT_010393.16 NG_009873.1
Ensembl:   ENSG00000005339
Entrez:   1387
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.620000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download