PrimePCR™ SYBR® Green Assay: FGFR2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033242

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021928
Assay Design:   Intron-spanning
Chromosome Location:   10:123258009-123260382question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000358487 ENST00000356226 ENST00000369060 ENST00000369059 ENST00000429361 ENST00000346997 ENST00000457416 ENST00000360144 ENST00000369056 ENST00000369058 ENST00000336553 ENST00000357555 ENST00000369062 ENST00000369061 ENST00000351936

Gene Information

The protein encoded by this gene is a member of the fibroblast growth factor receptor family where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region composed of three immunoglobulin-like domains a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors setting in motion a cascade of downstream signals ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic basic and/or keratinocyte growth factor depending on the isoform. Mutations in this gene are associated with Crouzon syndrome Pfeiffer syndrome Craniosynostosis Apert syndrome Jackson-Weiss syndrome Beare-Stevenson cutis gyrata syndrome Saethre-Chotzen syndrome and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Jan 2009]

Gene Symbol:   FGFR2
Gene Name:   fibroblast growth factor receptor 2
Aliases:   BEK, BFR-1, CD332, CEK3, CFD1, ECT1, FLJ98662, JWS, K-SAM, KGFR, TK14, TK25
RefSeq:   NC_000010.10 NG_012449.1 NT_030059.13
Ensembl:   ENSG00000066468
Entrez:   2263
Chromosome Mapping:   10q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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