PrimePCR™ SYBR® Green Assay: DUSP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033251

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DUSP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DUSP3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021960
Assay Design:   Intron-spanning
Chromosome Location:   17:41847109-41852145question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000226004 ENST00000397937

Gene Information

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK SAPK/JNK p38) which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases different tissue distribution and subcellular localization and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues mutation screening in breast cancer pedigrees and in sporadic tumors was negative leading to the conclusion that this gene is not BRCA1. [provided by RefSeq Jul 2008]

Gene Symbol:   DUSP3
Gene Name:   dual specificity phosphatase 3
Aliases:   VHR
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000108861
Entrez:   1845
Chromosome Mapping:   17q21

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