PrimePCR™ SYBR® Green Assay: LETMD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: LETMD1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: LETMD1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0022085
Assay Design:		Intron-spanning
Chromosome Location:		12:51450209-51451860question
Amplicon Length:		93
Splice Variants Targeted:		ENST00000551477 ENST00000550929 ENST00000262055 ENST00000550814 ENST00000547660 ENST00000418425 ENST00000547008 ENST00000552739 ENST00000551931 ENST00000380123

Gene Information

This gene encodes a mitochondrial outer membrane protein. It has a potential role in tumorigenesis which may result from negative regulation of the p53 tumor suppressor gene. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:		LETMD1
Gene Name:		LETM1 domain containing 1
Aliases:		1110019O13Rik, DKFZp586A011, HCCR-1, HCCR-2, HCCR1, HCRR-2
RefSeq:		NC_000012.11 NT_029419.12
Ensembl:		ENSG00000050426
Entrez:		25875
Chromosome Mapping:		12q13.12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998500
y-intercept	34.740000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.