PrimePCR™ SYBR® Green Assay: TBC1D1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033287

List Price:    $188.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TBC1D1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TBC1D1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022095
Assay Design:   Intron-spanning
Chromosome Location:   4:38134815-38138828question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000508802 ENST00000261439 ENST00000510573 ENST00000454732

Gene Information

TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2 (USP6; MIM 604334) yeast Bub2 and CDC16 (MIM 603461) (White et al. 2000 [PubMed 10965142]).[supplied by OMIM Mar 2008]

Gene Symbol:   TBC1D1
Gene Name:   TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
Aliases:   KIAA1108, TBC, TBC1
RefSeq:   NC_000004.11 NT_016297.16
Ensembl:   ENSG00000065882
Entrez:   23216
Chromosome Mapping:   4p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.250000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download