PrimePCR™ SYBR® Green Assay: MOCS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0033358


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PrimePCR™ PreAmp for SYBR® Green Assay: MOCS1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MOCS1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0022264
Assay Design:		Intron-spanning
Chromosome Location:		6:39881535-39883859question
Amplicon Length:		63
Splice Variants Targeted:		ENST00000373186 ENST00000373188 ENST00000373195 ENST00000340692 ENST00000425303 ENST00000432280 ENST00000341481 ENST00000308559 ENST00000373175

Gene Information

Molybdenum cofactor biosynthesis is a conserved pathway leading to the biological activation of molybdenum. The protein encoded by this gene is involved in this pathway. This gene was originally thought to produce a bicistronic mRNA with the potential to produce two proteins (MOCS1A and MOCS1B) from adjacent open reading frames. However only the first open reading frame (MOCS1A) has been found to encode a protein from the putative bicistronic mRNA whereas additional splice variants whose full-length natures have yet to be determined are likely to produce a fusion between the two open reading frames. This gene is defective in patients with molybdenum cofactor deficiency type A. A related pseudogene has been identified on chromosome 16. [provided by RefSeq Jan 2010]

Gene Symbol:		MOCS1
Gene Name:		molybdenum cofactor synthesis 1
Aliases:		KIAA0381, MIG11, MOCOD
RefSeq:		NC_000006.11 NG_009297.1 NT_007592.15
Ensembl:		ENSG00000124615
Entrez:		4337
Chromosome Mapping:		6p21.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	35.000000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.