PrimePCR™ SYBR® Green Assay: TMPRSS3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033443

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TMPRSS3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TMPRSS3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022480
Assay Design:   Intron-spanning
Chromosome Location:   21:43805584-43808605question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000291532 ENST00000433957 ENST00000398405 ENST00000398397 ENST00000380399

Gene Information

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a serine protease domain a transmembrane domain an LDL receptor-like domain and a scavenger receptor cysteine-rich domain. Serine proteases are known to be involved in a variety of biological processes whose malfunction often leads to human diseases and disorders. This gene was identified by its association with both congenital and childhood onset autosomal recessive deafness. This gene is expressed in fetal cochlea and many other tissues and is thought to be involved in the development and maintenance of the inner ear or the contents of the perilymph and endolymph. This gene was also identified as a tumor-associated gene that is overexpressed in ovarian tumors. Alternatively spliced transcript variants have been described. [provided by RefSeq Jan 2012]

Gene Symbol:   TMPRSS3
Gene Name:   transmembrane protease, serine 3
Aliases:   DFNB10, DFNB8, ECHOS1, TADG12
RefSeq:   NC_000021.8 NG_011629.1 NT_011515.12
Ensembl:   ENSG00000160183
Entrez:   64699
Chromosome Mapping:   21q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.310000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download