PrimePCR™ SYBR® Green Assay: SLC6A16, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033684

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC6A16, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC6A16, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0023095
Assay Design:   Intron-spanning
Chromosome Location:   19:49797702-49812175question
Amplicon Length:   122
Splice Variants Targeted:   ENST00000335875 ENST00000454748

Gene Information

SLC6A16 shows structural characteristics of an Na(+)- and Cl(-)-dependent neurotransmitter transporter including 12 transmembrane (TM) domains intracellular N and C termini and large extracellular loops containing multiple N-glycosylation sites.[supplied by OMIM Mar 2008]

Gene Symbol:   SLC6A16
Gene Name:   solute carrier family 6, member 16
Aliases:   NTT5
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000063127
Entrez:   28968
Chromosome Mapping:   19q13.1-q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 36.740000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download