PrimePCR™ SYBR® Green Assay: PTPRE, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033745

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PTPRE, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PTPRE, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0023302
Assay Design:   Intron-spanning
Chromosome Location:   10:129861394-129866396question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000254667 ENST00000306042 ENST00000439034 ENST00000419012 ENST00000430713

Gene Information

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. Two alternatively spliced transcript variants of this gene have been reported one of which encodes a receptor-type PTP that possesses a short extracellular domain a single transmembrane region and two tandem intracytoplasmic catalytic domains; Another one encodes a PTP that contains a distinct hydrophilic N-terminus and thus represents a nonreceptor-type isoform of this PTP. Studies of the similar gene in mice suggested the regulatory roles of this PTP in RAS related signal transduction pathways cytokines induced SATA signaling as well as the activation of voltage-gated K+ channels. [provided by RefSeq Jul 2008]

Gene Symbol:   PTPRE
Gene Name:   protein tyrosine phosphatase, receptor type, E
Aliases:   DKFZp313F1310, FLJ57799, FLJ58245, HPTPE, PTPE, R-PTP-EPSILON
RefSeq:   NC_000010.10 NT_008818.16
Ensembl:   ENSG00000132334
Entrez:   5791
Chromosome Mapping:   10q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.780000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download