PrimePCR™ SYBR® Green Assay: MAD2L2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0039164


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PrimePCR™ PreAmp for SYBR® Green Assay: MAD2L2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MAD2L2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0036440
Assay Design:		Intron-spanning
Chromosome Location:		1:11737629-11740464question
Amplicon Length:		68
Splice Variants Targeted:		ENST00000235310 ENST00000376672 ENST00000376669 ENST00000376667 ENST00000445656 ENST00000456915 ENST00000376692

Gene Information

The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein which is similar to MAD2L1 is capable of interacting with ADAM9 ADAM15 REV1 and REV3 proteins. [provided by RefSeq Jul 2008]

Gene Symbol:		MAD2L2
Gene Name:		MAD2 mitotic arrest deficient-like 2 (yeast)
Aliases:		MAD2B, REV7
RefSeq:		NC_000001.10 NT_021937.19
Ensembl:		ENSG00000116670
Entrez:		10459
Chromosome Mapping:		1p36

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998400
y-intercept	35.950000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.