PrimePCR™ SYBR® Green Assay: DLG2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039357

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DLG2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DLG2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0036633
Assay Design:   Intron-spanning
Chromosome Location:   11:83252844-83344309question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000398309 ENST00000426717 ENST00000376104 ENST00000418306 ENST00000404783 ENST00000330014 ENST00000524982 ENST00000532653 ENST00000531015 ENST00000420775 ENST00000524601 ENST00000398304 ENST00000530800 ENST00000529399 ENST00000434967 ENST00000543673 ENST00000280241 ENST00000537455 ENST00000376106

Gene Information

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. The encoded protein forms a heterodimer with a related family member that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors ion channels and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq Dec 2008]

Gene Symbol:   DLG2
Gene Name:   discs, large homolog 2 (Drosophila)
Aliases:   DKFZp781D1854, DKFZp781E0954, FLJ37266, MGC131811, PSD-93, PSD93, chapsyn-110
RefSeq:   NC_000011.9 NG_021375.1 NT_167190.1
Ensembl:   ENSG00000150672
Entrez:   1740
Chromosome Mapping:   11q14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.160000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download