PrimePCR™ SYBR® Green Assay: GDF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039806

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GDF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GDF1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037083
Assay Design:   Intron-spanning
Chromosome Location:   19:18981379-18989830question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000350527 ENST00000308713 ENST00000537485 ENST00000264057 ENST00000409813 ENST00000526712 ENST00000587869 ENST00000590469 ENST00000535453 ENST00000233607 ENST00000238483 ENST00000453981 ENST00000469391 ENST00000382331 ENST00000382329 ENST00000432829 ENST00000299308 ENST00000535886 ENST00000455537 ENST00000286452 ENST00000335025 ENST00000398831 ENST00000406385 ENST00000412327 ENST00000456792 ENST00000492445 ENST00000262803 ENST00000599848 ENST00000427170 ENST00000247005 ENST00000369519 ENST

Gene Information

This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis. This protein is transcribed from a bicistronic mRNA that also encodes the longevity assurance gene. [provided by RefSeq Jul 2008]

Gene Symbol:   GDF1
Gene Name:   growth differentiation factor 1
Aliases:   Not Available
RefSeq:   NC_000019.9 NG_003110.1 NG_012070.1 NT_011295.11
Ensembl:   ENSG00000130283
Entrez:   2657
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.580000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download