PrimePCR™ SYBR® Green Assay: RSPH10B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0039835


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PrimePCR™ PreAmp for SYBR® Green Assay: RSPH10B, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: RSPH10B, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0037112
Assay Design:		Intron-spanning
Chromosome Location:		7:5966127-5967866question
Amplicon Length:		63
Splice Variants Targeted:		ENST00000548523 ENST00000341698 ENST00000371650 ENST00000371652 ENST00000557021 ENST00000535557 ENST00000554546 ENST00000356296 ENST00000557597 ENST00000555238 ENST00000556772 ENST00000555394 ENST00000559312 ENST00000560335 ENST00000555738 ENST00000554521 ENST00000355058 ENST00000359560 ENST00000454166 ENST00000544991 ENST00000535282 ENST00000423334 ENST00000219313 ENST00000275162 ENST00000405415 ENST00000404406 ENST00000337579 ENST00000441023 ENST00000539903 ENST00000544099 ENST00000396908 ENST

Gene Information

Description Not Available

Gene Symbol:		RSPH10B
Gene Name:		radial spoke head 10 homolog B (Chlamydomonas)
Aliases:		MGC50833, RSPH10B2
RefSeq:		NC_000007.13 NT_007819.17
Ensembl:		ENSG00000155026
Entrez:		222967
Chromosome Mapping:		7p22.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	35.970000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.