PrimePCR™ SYBR® Green Assay: HLA-DRB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039968

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HLA-DRB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HLA-DRB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037245
Assay Design:   Intron-spanning
Chromosome Location:   6:32546835-32548601question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000334122 ENST00000564373 ENST00000569393 ENST00000392975 ENST00000360793 ENST00000584031 ENST00000581573 ENST00000448884 ENST00000582034 ENST00000542042 ENST00000580691 ENST00000582997 ENST00000584291 ENST00000392976 ENST00000576599 ENST00000320865 ENST00000444645 ENST00000457451 ENST00000334000 ENST00000397835 ENST00000414786 ENST00000411959 ENST00000437784 ENST00000465829 ENST00000481893 ENST00000306682 ENST00000541662 ENST00000340291 ENST00000262940 ENST00000461209 ENST00000449970 ENST

Gene Information

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB) both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3 DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3 DRB4 and DRB5. There are 4 related pseudogenes: DRB2 DRB6 DRB7 DRB8 and DRB9. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-DRB1
Gene Name:   major histocompatibility complex, class II, DR beta 1
Aliases:   DRB1, DRw10, FLJ75017, FLJ76359, HLA-DR1B, HLA-DRB, HLA-DRB1*, SS1
RefSeq:   NC_000006.11 NG_002392.2 NG_002432.1 NG_002433.1 NT_007592.15 NT_113891.2 NT_167249.1
Ensembl:   ENSG00000196126
Entrez:   3123
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.600000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download