PrimePCR™ SYBR® Green Assay: SYNGAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0039986


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PrimePCR™ PreAmp for SYBR® Green Assay: SYNGAP1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SYNGAP1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0037263
Assay Design:		Intron-spanning
Chromosome Location:		6:33406616-33408574question
Amplicon Length:		120
Splice Variants Targeted:		ENST00000249269 ENST00000428154 ENST00000357162 ENST00000358544 ENST00000395996 ENST00000455687 ENST00000395071 ENST00000414753 ENST00000551507 ENST00000329654 ENST00000341517 ENST00000423207 ENST00000371045 ENST00000418600 ENST00000449372 ENST00000428982 ENST00000293748 ENST00000231368

Gene Information

The protein encoded by this gene is a major component of the postsynaptic density (PSD) a group of proteins found associated with NMDA receptors at synapses. The encoded protein is phosphorylated by calmodulin-dependent protein kinase II and dephosphorylated by NMDA receptor activation. Defects in this gene are a cause of mental retardation autosomal dominant type 5 (MRD5). [provided by RefSeq Dec 2009]

Gene Symbol:		SYNGAP1
Gene Name:		synaptic Ras GTPase activating protein 1
Aliases:		DKFZp761G1421, KIAA1938, MRD5, RASA1, RASA5, SYNGAP
RefSeq:		NC_000006.11 NG_016137.1 NT_007592.15 NT_167249.1
Ensembl:		ENSG00000197283
Entrez:		8831
Chromosome Mapping:		6p21.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	35.390000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.