PrimePCR™ SYBR® Green Assay: C6orf27, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040039

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: C6orf27, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: C6orf27, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037316
Assay Design:   Intron-spanning
Chromosome Location:   6:31741167-31743126question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000455970 ENST00000377721 ENST00000394008 ENST00000391355 ENST00000571104 ENST00000570825 ENST00000576511 ENST00000452329 ENST00000551851 ENST00000547336 ENST00000368491 ENST00000434604 ENST00000360290 ENST00000392500 ENST00000419517 ENST00000368488 ENST00000374869 ENST00000330258 ENST00000403336 ENST00000359373 ENST00000393609 ENST00000430726 ENST00000550381 ENST00000552452 ENST00000438725 ENST00000547402 ENST00000550140 ENST00000438415 ENST00000546928 ENST00000551633 ENST00000375688 ENST

Gene Information

Description Not Available

Gene Symbol:   C6orf27
Gene Name:   chromosome 6 open reading frame 27
Aliases:   FLJ25524, G7c, NG37
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167247.1 NT_167249.1
Ensembl:   ENSG00000204396
Entrez:   80737
Chromosome Mapping:   6p21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.300000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download