PrimePCR™ SYBR® Green Assay: MICA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040062

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MICA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MICA, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037339
Assay Design:   Intron-spanning
Chromosome Location:   6:31380102-31382871question
Amplicon Length:   189
Splice Variants Targeted:   ENST00000393930 ENST00000310706 ENST00000393931 ENST00000373625 ENST00000438928 ENST00000415525 ENST00000414473 ENST00000423443 ENST00000546529 ENST00000420259 ENST00000418465 ENST00000432479 ENST00000417943 ENST00000552236 ENST00000449934 ENST00000421350 ENST00000318524 ENST00000283415 ENST00000358625 ENST00000425041 ENST00000417899 ENST00000427477 ENST00000446505 ENST00000420744 ENST00000547767 ENST00000400322 ENST00000400325 ENST00000436191 ENST00000547609 ENST00000405142 ENST00000422452 ENST

Gene Information

MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq Nov 2010]

Gene Symbol:   MICA
Gene Name:   MHC class I polypeptide-related sequence A
Aliases:   FLJ36918, FLJ60820, MGC111087, MGC21250, MIC-A, PERB11.1
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167246.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204520
Entrez:   100507436
Chromosome Mapping:   6p21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.250000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download