PrimePCR™ SYBR® Green Assay: DEFA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040107

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DEFA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DEFA1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037384
Assay Design:   Intron-spanning
Chromosome Location:   8:6833998-6836156question
Amplicon Length:   182
Splice Variants Targeted:   ENST00000354232 ENST00000419701 ENST00000244007 ENST00000373271 ENST00000373272 ENST00000382692 ENST00000382689 ENST00000535841 ENST00000327857

Gene Information

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine respiratory tract urinary tract and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene defensin alpha 1 is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin alpha 3 by only one amino acid. This gene and the gene encoding defensin alpha 3 are both subject to copy number variation. [provided by RefSeq May 2010]

Gene Symbol:   DEFA1
Gene Name:   defensin, alpha 1
Aliases:   DEF1, DEFA1B, DEFA2, HNP-1, HP-1, HP1, MGC138393, MRS
RefSeq:   NC_000008.10 NT_023736.17
Ensembl:   ENSG00000206047
Entrez:   1667
Chromosome Mapping:   8p23.1

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