PrimePCR™ SYBR® Green Assay: AMY2A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040230

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AMY2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AMY2A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037507
Assay Design:   Intron-spanning
Chromosome Location:   1:104160584-104161623question
Amplicon Length:   200
Splice Variants Targeted:   ENST00000361355 ENST00000414303 ENST00000423678 ENST00000430659 ENST00000370083 ENST00000370080 ENST00000446703 ENST00000330330 ENST00000370079

Gene Information

Amylases are secreted proteins that hydrolyze 14-alpha-glucoside bonds in oligosaccharides and polysaccharides and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq Jul 2008]

Gene Symbol:   AMY2A
Gene Name:   amylase, alpha 2A (pancreatic)
Aliases:   AMY2, AMY2B, PA
RefSeq:   NC_000001.10 NG_004750.1 NT_032977.9
Ensembl:   ENSG00000243480
Entrez:   279
Chromosome Mapping:   1p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.310000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download