PrimePCR™ SYBR® Green Assay: TRIM39-RPP21, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040243

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TRIM39-RPP21, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRIM39-RPP21, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037520
Assay Design:   Intron-spanning
Chromosome Location:   6:30297490-30303594question
Amplicon Length:   197
Splice Variants Targeted:   ENST00000552257 ENST00000546617 ENST00000552594 ENST00000550063 ENST00000552520 ENST00000426384 ENST00000426469 ENST00000419790 ENST00000443109 ENST00000450421 ENST00000452705 ENST00000296452 ENST00000521776 ENST00000458607 ENST00000431871 ENST00000446397 ENST00000431272 ENST00000424575 ENST00000412022 ENST00000549841 ENST00000550432 ENST00000552805 ENST00000549051 ENST00000550581 ENST00000400644 ENST00000447077 ENST00000383601 ENST00000383603 ENST00000383602 ENST00000457154 ENST00000550282 ENST

Gene Information

Description Not Available

Gene Symbol:   TRIM39-RPP21
Gene Name:   TRIM39-RPP21 readthrough
Aliases:   TRIM39R
RefSeq:   Not Available
Ensembl:   ENSG00000248167
Entrez:   202658
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.920000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download