PrimePCR™ SYBR® Green Assay: NOP16, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040473

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NOP16, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NOP16, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037750
Assay Design:   Intron-spanning
Chromosome Location:   5:175812246-175813863question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000510123 ENST00000389158 ENST00000509257

Gene Information

NOP16 is transcriptionally regulated by c-Myc (MYC; MIM 190080) upregulated in breast cancer and overexpression is associated with poor patient survival (Butt et al. 2008).[supplied by OMIM Jun 2009]

Gene Symbol:   NOP16
Gene Name:   NOP16 nucleolar protein homolog (yeast)
Aliases:   HSPC111, HSPC185
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000048162
Entrez:   51491
Chromosome Mapping:   5q35.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.790000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download