PrimePCR™ SYBR® Green Assay: CBWD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040896

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CBWD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CBWD1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038173
Assay Design:   Intron-spanning
Chromosome Location:   9:163978-172151question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000259199 ENST00000416503 ENST00000433343 ENST00000356521 ENST00000382447 ENST00000314367 ENST00000377447 ENST00000431099 ENST00000377400 ENST00000377457 ENST00000377449 ENST00000382405 ENST00000377395 ENST00000430059 ENST00000377392 ENST00000360171 ENST00000377344 ENST00000578449 ENST00000581344

Gene Information

Description Not Available

Gene Symbol:   CBWD1
Gene Name:   COBW domain containing 1
Aliases:   Not Available
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000172785
Entrez:   55871
Chromosome Mapping:   9p24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.280000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download