PrimePCR™ SYBR® Green Assay: SLIT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0040976


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PrimePCR™ PreAmp for SYBR® Green Assay: SLIT1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SLIT1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0038254
Assay Design:		Intron-spanning
Chromosome Location:		10:98820458-98823262question
Amplicon Length:		108
Splice Variants Targeted:		ENST00000453547 ENST00000399151 ENST00000393930 ENST00000310706 ENST00000393931 ENST00000266058 ENST00000371070 ENST00000314867 ENST00000371041 ENST00000448521 ENST00000440166 ENST00000452943 ENST00000468694 ENST00000494774 ENST00000432854 ENST00000490734 ENST00000562070 ENST00000341514 ENST00000343533 ENST00000350425 ENST00000341511 ENST00000265662 ENST00000371605 ENST00000561976 ENST00000389386 ENST00000424260 ENST00000263816 ENST00000448484 ENST00000249749 ENST00000343813 ENST00000264775 ENST

Gene Information

Description Not Available

Gene Symbol:		SLIT1
Gene Name:		slit homolog 1 (Drosophila)
Aliases:		MEGF4, MGC164811, SLIL1, SLIT-1, SLIT3
RefSeq:		NC_000010.10 NT_030059.13
Ensembl:		ENSG00000187122
Entrez:		6585
Chromosome Mapping:		10q23.3-q24

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	37.140000
Efficiency	93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.